Biology

Water Potential and Osmosis

Use water potential, tonicity, and membrane permeability to predict water movement and its effects on animal, plant, and protist cells.

Lesson progress
Define water potential and osmosisDistinguish hypertonic, hypotonic, and isotonic descriptionsPredict animal and plant cell responses to external solutionsExplain turgor pressure and contractile vacuoles

Water movement across membranes is best predicted using water potential: water moves spontaneously from a region of higher water potential to a region of lower water potential when a pathway is available.

Water potential model
Psi = Psi_s + Psi_p

Solute potential (Psi_s) becomes more negative as solute concentration increases. Pressure potential (Psi_p) represents physical pressure on water and is especially important in plant cells.

Definition

Osmosis

The net movement of water across a selectively permeable membrane from higher water potential to lower water potential.

Tonicity

Tonicity compares the effective concentration of non-penetrating solutes on two sides of a membrane. A hypertonic solution has greater effective solute concentration than the comparison solution; a hypotonic solution has less; isotonic solutions produce no net osmotic change in cell volume.

Cell responses to external solutions
External solutionAnimal cellPlant cell
HypotonicWater enters; the cell swells and may lyse if the imbalance is extreme.Water enters; the vacuole expands and turgor pressure rises. The wall resists further expansion, producing a turgid cell.
IsotonicNo net long-term volume change.Low turgor; cell is typically flaccid.
HypertonicWater leaves; the cell shrinks/crenates.Water leaves; the protoplast can pull away from the wall (plasmolysis).

Turgor pressure

As water enters a plant cell, the plasma membrane and cytoplasm press against the cell wall. The wall pushes back, increasing pressure potential. At equilibrium the water-potential difference is balanced and there is no net water movement.

Contractile vacuoles

Many freshwater protists continuously gain water by osmosis because their surroundings are very dilute. A contractile vacuole collects excess water and periodically expels it, helping maintain cell volume and osmotic balance.

Key idea

Tonicity and water potential are related but not identical ideas. Water potential includes both solute effects and pressure, so it is the more complete framework for predicting water movement in plant tissues.